Oct 11, 2013

Additional sample collection and DNA extraction

Goals: take additional samples from Otto's and from commercial vendors.
Methods: our group had lunch at Otto's while Chris took five additional samples from completed beer. After, we purchased two of these beers in bottles from a local store. Back in lab, up to 120 ml of all samples were filtered through Sterivex millipore filters. This makes for 7 samples today and 11 samples overall.

Goals: extract DNA
Methods: the Mo Bio kit was used to extract DNA from the filters after filters were sliced into thin strips. A Qubit® 2.0 Fluorometer was used to measure DNA concentrations in extracted samples.
Results: Of the 11 samples, only 3 produced delectable quantities of DNA (>5 ng/ml).

Next: get all possible DNA from existing samples

15 hours over two days

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